As you can see, it does not resemble the original strand at all. The complementary strand must be the palindrome sequence flipped.īack to the “false” palindrome sequence, it’s complimentary strand is “TAAT”. Therefore, this is not a palindrome sequence!!! This is because a restriction enzyme cuts through the complementary strand and the strand we see in the sequence. For example, “ATTA” follows that mirror copy tule I was talking about, BUT the first BP (A) is the same as the last BP (A). One thing I wanted to mention also is that the first Bp in the sequence CANNOT be the same as the last Bp in the sequence. As you can see, AAG is a “mirror copy” of CTT. In this question, we have a 6 Bp palindrome sequence. The key to finding these sequences is knowing that they are usually 4 or 6 base pairs in length, and a trick I like to use is that the first “half” of the sequence is a mirror copy of the second “half” of the sequence. In this case, we are talking about southern blot so restriction enzymes are involved. You should be looking for palindrome sequences whenever you get asked a question that involves restriction enzymes.
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